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To confirm the neuronal enrichment of the isolated mRNA, cDNA was used to measure the relative expression of neuron-specific MAP2 (microtubule associated protein 2) and TUBB3 (III-tubulin), microglia/macrophage-specific Iba1 (ionized calcium binding adaptor molecule 1) and CD13, astrocyte-specific GFAP (glial fibrillary acidic protein), and oligodendrocyte-specific MBP (myelin basic protein) transcripts, using the CT method